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The mechanism related to particulate matter pollution is still unclear, although the evidence presented in this study indicates the hypothesis that oxidative stress could be a responsible factor behind the harmful effects of PM2.5 on sperm parameters and fertilization. Exposure to high concentrations of PM2.5 disturbs various stages of spermatogenesis, damages the basement membrane and tunica propria as well as reduces the number of germ cells . These may cause damage to the arteries that supply blood to the penis and reduce the blood flow in the penis . Lead-induced oxidative stress can change the expression of antioxidant enzymes, such as superoxide dismutase 2 (SOD2) and galactoside acetyltransferase (GAT) that, in turn, decreases progesterone production . Total RNA was extracted from ∼15 mg of muscle using a TRIzol/ethanol precipitation method. A total of three muscle biopsies were collected from one incision on one leg per biopsy protocol day using a 5-mm Bergström needle with manual suction (22) and under local anesthesia (1% lidocaine). An absolute intensity was used to limit the confounding effects of weight loss on relative exercise intensity and standardize the absolute stress. Percutaneous muscle biopsies of the vastus lateralis were collected at rest and after a steady-state aerobic exercise bout on day 14 of WM and following the 28-day ED on day 42. Available muscle tissue was also a consideration when selecting individuals for analysis. Learn about steps to take, as well as things to avoid, to keep sperm healthy and improve fertility. But a variety of factors can affect sperm health. Having healthy sperm is an important part of fertility. Understand how lifestyle can affect sperm health and find out what you can do to boost fertility. 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